How to align injection and holding pipettes accurately ?

Co-Planar Focus: Bring both tips into the same focal plane, then lock Z-axis before adjusting X/Y alignment.

Back-Light Contrast: Use oblique illumination to create silhouette outlines—overlap the tips precisely.

Test Grasp: Gently approach a microbead in the drop and ensure both pipettes meet at the bead’s center, then back off slightly to confirm symmetrical contact.