FAQ

What’s the link between injection depth and fertilization ?

  • Too Shallow: Sperm may lodge in the zona or perivitelline space, failing to trigger activation.

  • Too Deep: Risks spindle contact or cytoplasmic leakage, compromising embryo development.

  • Optimal: Penetrate just past the oolemma—typically 2–3 µm into the ooplasm.

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