FAQ

How to prevent sperm tail curling inside the micropipette ?

  • Pipette Diameter: Use a tip inner diameter (~5 µm) just large enough for the head but snug enough to align the tail outward.

  • Gentle Aspiration: Apply slow, consistent suction—rapid drawing can loop the tail.

  • Straighten in PVP: Rotate or gently tease the sperm in the PVP drop so the tail extends along the pipette axis.

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