FAQs on Micropipette Handling and Setup

Should I use pre-polished or self-polished pipettes ?

How to align injection and holding pipettes accurately ?

What are common causes of air bubbles in pipette systems ?

What happens if the pipette inner diameter is too small for sperm ?

How to mount micropipettes without introducing vibration ?

How to avoid tip breakage during zona penetration ?

How to calibrate micromanipulators before starting ICSI ?