What’s the link between injection depth and fertilization ?

Question

Accepted Answer

Too Shallow: Sperm may lodge in the zona or perivitelline space, failing to trigger activation.
Too Deep: Risks spindle contact or cytoplasmic leakage, compromising embryo development.
Optimal: Penetrate just past the oolemma—typically 2–3 µm into the ooplasm.

What’s the link between injection depth and fertilization ?

Holding Micropipette

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