How to calibrate micromanipulators before starting ICSI ?

• Zero Reference Point: Park both injection and holding pipettes in designated “home” wells, then set X/Y/Z coordinates to zero.

• Movement Check: Step through full range of motion (X, Y, Z axes) at low magnification to confirm linear and smooth traverse.

• Fine-tune under High Magnification: Switch to ICSI settings (400–600×) and make micro-adjustments until pipette tips remain in focus throughout their travel.

• Record Settings: Log joystick sensitivity and speed parameters for reproducibility across sessions.