What techniques ensure consistent penetration of the oolemma ?
Bevel Sharpness: Maintain a 10–15° bevel for a defined cutting edge.
Pre-drills or Piezo: In cases of tough membranes, use a brief laser pulse or piezo pulse to weaken the oolemma first.
Depth Control: Retract the tip 1–2 µm after crossing the zona to position just outside the oolemma, then advance into the cytoplasm.
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