What causes micropipette tip breakage during ICSI, and how to avoid it ?

Common Causes :

• Sudden pressure changes during sperm injection

• Excessive force while penetrating the zona pellucida

• Manufacturing defects or micro-fractures from handling

Avoidance Tips :

• Use micro-pipettes designed specifically for ICSI with reinforced tapering

• Control injection pressure using microinjector settings carefully

• Minimize contact between the micro-pipettes and the ICSI dish base

• Handle micro-pipettes with delicate forceps—never bare hands