What are the best practices for aligning the holding and injection pipettes ?

• Co-planar Focus: Bring both tips into focus at the same Z-height, then lock that plane.

• Back-light Contrast: Use oblique illumination to silhouette the tips and overlay them precisely in X/Y.

• Test Grip: On a microbead or oil droplet, gently close suction to ensure both tips meet centrally before switching to oocytes.