How to troubleshoot polyspermy after ICSI ?

• Verify Single Sperm Aspiration: Watch under high magnification that only one sperm enters the pipette.

• Optimize PVP Concentration: Use minimal viscosity to slow but not trap multiple sperm.

• Clean Pipette Tip: Back‐flush between injections to remove residual sperm.

• Adjust Holding Suction: Too weak allows stray sperm into the perivitelline space.