How to deal with viscous sperm samples in ICSI ?
Dilution: Dilute with an appropriate culture medium (e.g., HEPES-buffered) to reduce viscosity.
Swim-up or Density Gradient: Pre-clear debris and seminal plasma to isolate motile sperm.
Adjust PVP: Use slightly lower–viscosity PVP for aspiration without compromising immobilization.
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