How to avoid tip breakage during zona penetration ?

• Optimize Bevel Angle: A 10–15° bevel provides a sharp entry point without weakening the glass wall.

• Controlled Speed: Advance at a steady, moderate speed—too rapid movement increases shear stress.

• Pressure Calibration: Set microinjector suction just high enough to hold the oocyte firmly but low enough to prevent sudden pressure spikes.

• Practice Entry Depth: Aim for penetration just past the zona, avoiding excess travel into the ooplasm.