How to identify and avoid over‐injection ?

  • Signs: Excessive cytoplasmic leakage, large, persistent media droplets at the tip, or oocyte collapse.

  • Prevention:

    • Calibrate injection pressure and duration (e.g., 1–2 s pulses).

    • Use the minimum volume needed to release the sperm.

    • Monitor each injection under real‐time imaging.

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