How can you prevent oocyte rotation during injection ?

Question

Accepted Answer

Stable Suction: Apply just enough negative pressure to grip the zona—avoid over-suction that deforms the oocyte.
Symmetric Hold: Center the pipette on the oocyte’s widest plane, not off to one side.
Oil Overlay: A thin oil layer damps fluid currents that can spin the oocyte when pipettes move.

How can you prevent oocyte rotation during injection ?

Holding Micropipette

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